The HP diets increased abundance of Bifidobacterium spp. Moreover, there was no difference in AID of CP and AA between HP and LP diets, but SID of cystine, glutamic acid, glycine, and proline was lower ( P < 0.05) in pigs fed the HP diets. did not affect both AID and SID of CP and AA in growing pigs. Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS. Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR. The apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA was determined. The LP and HP diets were supplemented with or without Bacillus spp. The assay diets were based on wheat-barley-soybean meal with two protein levels: LP (14% CP, as-fed) and HP diet (18% CP, as-fed). MethodsĮight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a row-column design with 8 pigs and 3 periods of 16 d each. supplementation to low- (LP) and high-protein diets (HP) on ileal CP and amino acid (AA) digestibility and intestinal microbiota composition. Therefore, the objective of this study was to determine the effect of Bacillus spp. in combination with different dietary crude protein (CP) levels on the ileal digestibility and microbiota composition. However, there is little information on the effect of Bacillus spp. seem to be an alternative to antimicrobial growth promoters for improving animals’ health and performance.
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